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EFFICACY OF SYSTEMIC ENZYMES IN SERRAQUIN ES15™

IN TREATING NON-SPECIFIC CASES OF

LAMENESS AMONG EQUINES (In Brief)

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Dr. Rakesh Pattu, BVSc & AH Study on File 9/20/2005

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1) A trial was conducted on six race horses suffering from non-specific lameness. The goal of the
study was to test the efficacy of the ingredients in Serraquin ES15™ in ameliorating their condition.

SERRAQUIN ES15™ was formulated for Steel Horse Formulations LLC

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2) All six clinically lame horses have been examined and the seat of lameness identified. All
horses were given the ingredients in Serraquin ES15™ powder at 10 grams orally three times daily for a period of 7 days.
No other analgesic, anti-inflammatory or moist dressings were used.

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3) Five out of six horses showed complete recovery and one horse continued to show lameness.

The results are as follows:

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Number             Name                          Diagnosis                                                 Remarks     
                         

1                        Flying Hawk               Tendinitis LF                                             Responded
 

2                        Heart Throb                Sprain Ligament Knee LF                       No  Response

3                        Power On                    Sprain Ligament Lumbar Region           Responded

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4                        Answer                        Sprain Muscle Should LF                        Responded
 

5                        Montgomery Hero      Tendinitis RF                                           Responded                   
 

6                        Picardo                        Sprain Ligament Stifle RH                      Responded
 

4) Hematological, urine and fecal examination was carried out during pre and post trial period. No
significant hematological, urine or fecal parameter changes were noted.

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Conclusion:

Since the results are quite encouraging, a comparative study on a larger number of animals to
further determine its efficacy on bone, tendon, ligament and muscle related conditions.

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Efficacy of the ingredients in Serraquin ES15  in Equine Lameness, An Observation (Abstract)

Study on File 4/18/2006

Dr. Surajit Basu BVSC & AH

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In a study of a 16-year-old gelding and a 7-year-old mare, both presenting with lameness, the
combination of proteolytic enzymes in Serraquin ES15™ demonstrated effectiveness in reducing pain &
inflammation. In addition, the product demonstrates fibrinolytic activity and improves microcirculation to the affected areas.